Effects of Reusing Gel Electrophoresis and Electrotransfer Buffers on Western Blotting
نویسندگان
چکیده
منابع مشابه
Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis.
Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. I...
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15 صفحه اولWestern Blotting
Histological Analysis and Immunohistochemistry Renal sections (3 μm thick) were stained with periodic acid-Schiff (PAS) and examined under light microscopy. Glomerulosclerosis index was semiquantitatively graded as 0 to 4+; vascular injury score as 0 to 3+; and tubulointerstitial injury score as 0 to 5+, according to the criteria reported previously. Ultrastructure of glomerular podocytes was a...
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ژورنال
عنوان ژورنال: Journal of Biomolecular Techniques : JBT
سال: 2016
ISSN: 1524-0215,1943-4731
DOI: 10.7171/jbt.16-2703-004